FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10675-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10675-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14437657
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT11136747.713675425313124TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCGCGTAT326050.22583304202336987TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1419300.067.9121615
GAGCACA1427000.067.883949
GATCGGA1416500.067.840911
CACGTCT1422700.067.8359614
GCACACG1423850.067.8278711
GTCCCGA1326450.067.60541536
AGCACAC1435500.067.48952510
TCGGAAG1437750.067.478983
GTCTGAA1427900.067.4538817
ACCCGTC1356000.067.37434432
AGAGCAC1439850.067.3558968
CGTCCCG1336550.067.3516235
TCCCGAT1323300.067.3484637
CCCGATC1314350.067.3011238
TCACCCG1364600.067.2829330
ACACGTC1437700.067.276713
CACCCGT1363100.067.2285931
CACACGT1438300.067.2218812
CGTCTGA1434600.067.18056516
TCCAGTC1423350.067.0434125
Produced by FastQC (version 0.11.5)