FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10517-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10517-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16495480
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC2357391.4291127023887755TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1159400.7028592074919917TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC528920.3206454131677284TruSeq Adapter, Index 5 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG494950.3000518930034167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC478000.064.7402745
CGTATGC479100.064.2994344
ACACGTC737200.064.0871313
CACACGT737050.064.0241912
ACGTCTG736850.063.93688615
CACGTCT738000.063.85148214
GCACACG740850.063.67690311
GTCACAC725500.063.5352529
GATCGGA740900.063.2543261
TCCAGTC739000.063.0942925
CGTCTGA747050.063.08264516
AGTCACA739300.063.02626828
ACACAGT724050.062.9569332
GTCTGAA749150.062.95234317
CAGTCAC742750.062.8843127
TATGCCG481300.062.6726546
TCGTATG423650.062.66142743
CCAGTCA744300.062.65460226
GAGCACA754950.062.6218769
CACAGTG727500.062.37876533
Produced by FastQC (version 0.11.5)