FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10517-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10517-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10684564
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC7052736.600858958774546TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239130.2238088517229154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG864250.067.60058615
GTCACGC849700.067.5614329
CACGTCT869250.067.3285114
GCACACG870800.067.3215211
ACACGTC870900.067.2896713
AGTCACG861800.067.2220328
CACACGT872200.067.1813512
TCACGCC848800.067.1258830
GTATGCC802850.067.1030645
TATCTCG819300.067.0415639
CGTCTGA871800.066.9950716
GATCGGA865550.066.987651
TCCAGTC868600.066.7720225
CTCGTAT802000.066.7028642
CACGCCA853550.066.6703231
CCAGTCA870550.066.6147226
GAGCACA881300.066.6025169
AGCACAC881400.066.58732610
CGTATGC807900.066.575344
TATGCCG804650.066.5701946
Produced by FastQC (version 0.11.5)