FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17447666
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12770927.31955781363536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAT17250.056.0702321
CCTGGCC32700.050.4525835
CTTGTTA17050.046.8435331
ATACGCA20650.045.8290678
TGCGCGA28450.041.2702039
TGGCCCG40150.040.0039147
CTGGCCC46200.035.3956686
GCCCGGA51200.033.968579
TGAGTTA44900.032.5145768
CACCTGG52550.031.6602153
CGAACGC20250.031.4805071
ACCTGGC54700.030.6909664
GTGCGCG41700.029.9077858
GGCATAG34500.026.9918865
GCATAGT33500.026.9244966
CCAGACG15000.026.8880126
GCAAGTG23750.026.539673
AGTGATC24350.026.2003786
GTCCAAT7450.025.5719931
GACATCG57500.025.4931143
Produced by FastQC (version 0.11.5)