FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_T1cD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_T1cD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35643843
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA747460.20970241620691688No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT717070.20117639952571895No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA670020.1879763638281091No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT646040.18124869419944423No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC636170.1784796325132506No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC493130.1383492795656181No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT480960.13493494514606633No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG475390.1333722629178902No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG447620.12558129604599594No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC441670.12391200354013455No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC438230.12294689997372056No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC436610.12249240352674655No Hit
CTAGCTCAGAGAGGACGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTC434280.12183871419251847No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA432560.12135616240931148No Hit
CTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCT430460.12076700034841922No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC416930.11697111335609912No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG416430.1168308366749343No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA394900.11079052278397702No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC390960.10968514253639822No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG387910.10882945478129281No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT387360.1086751504320115No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG380300.1066944436939642No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT377350.10586681127509176No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC376850.10572653459392692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA105400.057.738914
TTACGAC105650.057.6821255
CAAACGT211600.055.4132421
AACGTTG217850.053.2971733
CGTTGAG227600.050.9054265
CCAGACG77000.050.8830266
AAACGTT234450.049.886032
GTGCGTC52750.049.8216869
CTTGTTA130000.047.6358261
TGGCCCG255200.043.1905257
TGAGTTA271700.043.0902828
CCGTATA18550.042.573536145
ACGACTT151200.040.6003657
CGCGGAT16900.040.332981
TACGCAA39550.038.548299
TGCGCGA262250.038.4243289
CCTGGCC288150.038.2949945
GTGCGCG264200.038.2695128
TTGAGTT318150.037.9062167
ATACGCA42600.036.1210988
Produced by FastQC (version 0.11.5)