FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_T1aD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_T1aD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35014434
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG658200.18797962006182936No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG635090.1813794848147481No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT582900.1664742031814651No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA577260.16486343888923066No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC571930.16334120951376796No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC571580.1632412507367676No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT568880.16247014017133618No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC527110.1505407741276069No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG471180.1345673615629486No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC461810.13189132230439596No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA446930.12764164629935185No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC441080.12597090674091718No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC426460.12179548582735909No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT414660.1184254470599182No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT382240.10916640834462725No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC381130.10884939622328323No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC355480.10152384585168504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT75600.052.51871145
TTACGAC104550.051.005775
GTTACGA106150.050.297334
AACGTTG200900.044.9825943
CAAACGT204450.044.584681
TACGCAA53850.044.3534249
CGTTGAG213550.042.2306335
TGTGCCG107750.041.757027145
AAACGTT220300.041.4083672
TGGCCCG234500.040.227677
ATACGCA60250.039.7544948
CTTGTTA141250.039.0177881
ACGACTT139200.038.5759667
GAAACTA314900.036.6739354
CGCGGAT16450.036.1480941
GAGTCTA140700.035.4066282
TGAGTTA261800.035.154348
TCTAACG125800.034.7770465
TGCGCGA249950.034.504869
AGTCTAA131500.033.4236033
Produced by FastQC (version 0.11.5)