FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_C2bD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_C2bD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34808155
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT1198700.34437332286069167No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1075260.3089103688489091No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC974880.2800722991494378No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA798070.22927673127173792No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC740630.21277485118070752No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT691180.19856841019008334No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC604060.17353979261469044No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC582620.16738031648043397No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC568520.16332954159736418No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG562190.16151100223496476No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG552940.15885357899607147No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA537530.15442645552457462No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC533020.1531307821399899No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC514020.14767229116280364No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT509070.14625021061874724No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACACGACCCGTC491130.14109624598028825No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT484750.13926334216794886No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT483190.138815171329822No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA468240.13452020079777283No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA457070.1313111826811849No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT454350.13052975660445088No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT453170.13019075558586773No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC443400.12738394206759882No Hit
CCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGTG438210.12589291216383058No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG436760.12547634311557163No Hit
CGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGCC433250.12446795872978617No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG425340.1221955027492839No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC425140.12213804494952404No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT413720.11885720458323633No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC398410.1144588100116194No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC396640.11395030848374468No Hit
AAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCA395170.11352799365550975No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC394320.11328379800653036No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC392760.1128356271684035No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT391510.11247651591990439No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT387900.11143940263423902No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGT387230.11124691900504351No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGTC382090.10977025355121522No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA357120.1025966472511973No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT356500.10241852807194177No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC351940.10110849023741705No Hit
CGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATGAAG349360.10036728462051493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT130250.053.723631
GTGCGTC55350.052.595659
AACGTTG132600.052.5060123
GAAACTA333500.049.8284574
AAACGTT141750.049.5156482
AGAAACT337750.049.430083
CGTTGAG143750.048.348255
AAGAAAC364550.046.0252762
TCGTGTT38100.045.449837145
CGGGCAT50000.041.483693
CGAACGC313100.040.969491
GAGTCTA170900.040.6031762
TGTGCCG60750.040.31158145
GTTACGA79000.040.0320244
TTACGAC79900.039.769535
AGTCTAA177150.038.8923153
ACGTTGA186200.038.682794
TGGCCCG143350.038.672587
CAGGCGT74000.037.849757
TCTAACG218650.037.837842
Produced by FastQC (version 0.11.5)