FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_C1bD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_C1bD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660828
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT125860.2700378559346107No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG118160.253517186216698No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC96260.20652982688912788No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT90670.19453624978222753No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC89930.19294854905609046No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC87780.18833563478420573No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC87360.18743450734504685No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC68740.1474845242090032No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG68010.1459182788980842No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA65280.1400609505435515No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC64440.13825869566523374No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG61510.13197225900633966No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC60810.13047037994107485No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT60300.12937615376495334No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC59500.12765972054750788No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA59080.126758593108349No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC59060.12671568227791286No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA57840.1240981216213085No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC52350.11231909866658886No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC51210.10987318133172903No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT50670.10871458890995335No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT47980.10294308221629289No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT47020.10088336235535832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTC7400.054.9362539
ATACGCA3950.053.2873658
TGTGTCG5050.051.7042853
CGCGGAT1551.4551915E-1146.789281
TTACGAC12350.046.4079255
GAAACTA41650.046.1507644
CAAACGT18200.045.028261
TACGCAA4550.044.6734359
CCGTATA1950.044.58621145
GAGTCTA22300.044.55092
GTTACGA13050.044.4659464
CTTGTTA13200.043.4041521
AACGTTG18750.043.324363
AGAAACT44850.043.178173
AGTCTAA21700.043.1166883
CGTTGAG18950.042.495785
AAGAAAC46900.041.5932
CGTTGTA2100.041.401478145
AAACGTT20050.040.508342
GGAGTCT24750.040.4372831
Produced by FastQC (version 0.11.5)