FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n02_C1aD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n02_C1aD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6117316
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT128900.21071332590959826No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA120220.19652409651553068No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA117940.19279697174381705No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT109050.17826445454182846No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG100360.16405887810928846No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG89700.1466329350976801No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC79590.13010607920205527No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC72230.11807465888634819No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA69940.11433118707616216No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC67670.11062040934292099No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT66700.10903474661109545No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC66500.1087078058416469No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC66380.10851164137997775No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG65740.10746543091774237No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC65470.1070240608789868No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC65370.10686059049426253No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG65370.10686059049426253No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG65300.1067461612249555No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT64150.10486625180062628No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG61500.10053428660543283No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA61180.10001118137431514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA23350.067.078081
CGCGGAT3300.063.7232361
CTAGCAT14950.062.084411
CCAGACG11300.057.1388446
TTACGAC27950.056.8408855
GTTACGA28150.056.68644
CCTGGCC38650.056.120335
GTGCGTC6150.055.473359
GTCCAAT8150.054.2732731
ATACGCA17550.052.515648
TACGCAA18200.051.4493569
CTCAGAC7650.048.3417661
TGGCCCG47050.045.9539387
TGTGTCG4250.044.3664863
GACAGGC7300.043.7248545
AACCTTG10350.042.755115
AGAAACT36800.041.1878473
CCGGCAT6700.041.1265751
GGTCTGG6350.041.1149143
GAAACTA37000.040.7752084
Produced by FastQC (version 0.11.5)