FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17447666
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16163769.264138825215934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAT17050.056.7823371
CTTGTTA16000.055.1301271
CCTGGCC36750.054.2502025
TGGCCCG45650.044.1429567
ATACGCA22900.043.9876638
TGCGCGA31350.041.933149
GTGCGCG32500.040.2267538
GCCCGGA58900.040.0290959
CCAGACG13400.038.533896
GTCCAAT7450.035.6163441
CTGGCCC56800.035.226626
CCGGCAT4950.034.770421
CACCTGG60850.034.409823
AGTCTAA12450.033.9815183
ACCTGGC63500.033.2029154
TCTAACG14800.032.9418952
TGAGTTA50900.032.141418
TGCAAGT27400.031.1385082
TCCACTC32750.031.0869732
ACTCCTG33300.031.0121695
Produced by FastQC (version 0.11.5)