FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_T1cD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_T1cD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35643843
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA789030.2213650194789602No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT720280.2020769758187971No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA713440.20015799082046234No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC667670.18731706342663446No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT656810.1842702539117345No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC506850.14219847169678085No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC497370.1395388258218958No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG495910.13912921791289454No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT494500.13873363767200972No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC473660.1328869056010599No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG458060.12851027314871744No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC446820.12535685335613223No Hit
CTAGCTCAGAGAGGACGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTC441050.12373806045549017No Hit
CTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCT439120.12319659246619395No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG423260.11874701613964578No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA417050.11700477975957868No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA411850.11554590227546452No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG407310.11427219001048793No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC404020.1133491694484234No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG396900.11135162950863631No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT395990.11109632594891634No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT384300.10781665714328278No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC380240.10667761049222442No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC361000.10127976380100204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA105250.059.854594
TTACGAC105600.059.724615
AACGTTG223200.053.62383
GTGCGTC52100.052.036949
CAAACGT235600.051.0280531
CGTTGAG237150.050.590955
AAACGTT237350.050.580642
CCAGACG77450.047.8296786
CTTGTTA134400.046.9374771
TGAGTTA279600.043.219168
ACGACTT149600.042.108147
TGGCCCG273300.041.5630347
TACGCAA37900.039.4008759
CCTGGCC294300.038.943915
GAAACTA222250.038.29364
TTGAGTT324550.038.238437
TGCGCGA267200.036.6790549
GTGCGCG270750.036.358778
ATACGCA42150.035.944018
GATGAAC259900.035.397172
Produced by FastQC (version 0.11.5)