FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_T1aD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_T1aD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35014434
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG687830.19644184452617452No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG684840.19558791097408573No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC621370.17746110075633378No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA609460.17405964637326424No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT599930.17133791167379717No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT581810.1661629029902354No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC544120.15539877068982466No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC515770.1473021097527951No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC502310.14345798078586677No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA487890.13933967917345172No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG485610.1386885191404208No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC482000.13765751575478843No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC473300.1351728261550651No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT450240.12858697073326958No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT415500.11866534812471907No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC403720.11530102128739252No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC395920.11307336854281295No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC365530.10439409073412412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC98300.055.975125
GTTACGA100000.054.9510464
TACGCAA53600.044.3593759
AACGTTG204350.044.3450623
ATACGCA55700.042.8170858
TGGCCCG218550.042.0906837
CAAACGT218250.042.0285871
CGTTGAG217800.041.9391335
ACGACTT133500.041.3760347
AAACGTT221200.041.2952652
GTGCGTC44050.040.8114629
GAAACTA307300.039.8685954
CTTGTTA139400.039.5849761
CCAGACG70800.038.7047356
GAGTCTA137150.035.3626822
TGAGTTA263900.035.0225078
TCTAACG126650.034.917212
AGAAACT364500.033.632253
TGCGCGA255300.033.476459
AGTCTAA128800.033.3208243