FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_C2bD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_C2bD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34808155
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT1214550.3489268534916602No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1119490.32161716126580103No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC1042290.299438450558497No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA796850.22892623869320278No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC789210.22673135074237633No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT722090.20744851314296892No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC684690.19670390458787604No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG603460.17336741921541088No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC583950.167762410848837No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA566630.16278656538963354No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG561500.16131277282579326No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC556960.16000848077124458No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC543210.1560582570377545No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT521810.14991027246345No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACACGACCCGTC520790.14961723768467478No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT519510.14924950776621168No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC513650.14756599423324793No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT496350.14259589455402047No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA481440.13831241558192325No Hit
CCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGTG481260.1382607035621394No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA477530.1371891155966181No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT475480.13660017314907957No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC474020.13618073121083263No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC472610.13577565372252565No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT470930.13529300820454288No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG458940.13184841310893955No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG448780.1289295568811389No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT439930.12638704924176533No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGT428120.12299416616594588No Hit
CGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGCC423290.12160656030174538No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT415760.11944327414078683No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC412930.11863024627418489No Hit
AAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCA409200.11755865830866358No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC407690.11712485192047668No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC407350.11702717366088493No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC400490.1150563711291219No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT400220.11497880309944611No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGTC398770.11456223405118715No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA383040.11004317810007454No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCC378090.10862109755601812No Hit
CTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCGCGTCGTTTCCC371430.10670775282401494No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT366370.10525407049009061No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG364570.10473695029225193No Hit
CCGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCCCGTCCGGGGGCCC363830.10452435643314045No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGA363720.10449275464327254No Hit
CGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATGAAG361270.1037888965962143No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG351410.10095622706805345No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT350800.10078098077878589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTC56350.055.5724689
AACGTTG128100.052.9119223
CAAACGT133500.050.838461
GAAACTA340550.050.3856124
AAACGTT135850.050.2149352
AGAAACT349150.049.248583
CGTTGAG138000.049.168185
GTTACGA70050.048.638364
CGGGCAT51750.048.328113
AAGAAAC361100.048.0416762
TTACGAC71600.047.4839865
GAGTCTA171200.042.42942
CTCACCC182400.041.692082
TCTAACG223300.039.8669132
AGTCTAA180000.039.7901463
CGAACGC301200.039.480861
TGTGTCG34250.039.1563533
CTTGTTA88650.038.8519331
ACGTTGA182750.038.7945484
GGAGTCT192100.038.0479241
Produced by FastQC (version 0.11.5)