FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_C2aD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_C2aD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4891544
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA159340.3257458176804706No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT143500.2933634042748057No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT115180.2354675742464956No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG111370.22767862253717844No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA84910.1735852728708972No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC70700.14453514064270914No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG69390.14185704963504364No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA67600.1381976733726611No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT67440.13787057828775537No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA63700.13022473067808446No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT62980.1287528027960088No Hit
CTAGCTCAGAGAGGACGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTC59260.1211478420719511No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG58820.12024833058846042No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC55600.11366554200473306No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC55470.11339977724824718No Hit
CTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCT55250.11295002150650184No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG54670.11176430182371865No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC53710.1098017313142844No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT53580.10953596655779853No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC53380.10912709770166638No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT53230.10882044605956728No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC53090.10853423786027479No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC52480.10728718784907179No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT50850.1039549066715949No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG50370.10297362141687778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTC6150.061.2931029
AACGTTG40100.059.2973183
CAAACGT40600.058.5784341
CTTGTTA16150.058.3661161
AAACGTT41200.057.712962
GTTACGA16650.057.4731834
CGTTGAG42900.055.4270975
TTACGAC17400.055.412535
GTCCAAT5250.053.8635861
CTCACCC19300.052.2100562
CCTGGCC34750.052.154385
CCAGACG12700.051.944946
TGAGTTA46200.051.1515778
CGCGGAT2450.050.3121381
CTCAGAC6950.049.034641
AACCTTG6400.048.7073025
TTGAGTT49150.048.2289287
GAAACTA38800.047.4577984
CCTCAAC8450.047.1949921
CCGGCAT3400.046.9173741
Produced by FastQC (version 0.11.5)