FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_C1cD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_C1cD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6913729
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT200250.2896410894902013No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA199000.28783309267690416No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT155530.22495819549768295No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC135720.19630506200054992No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA131990.19090999950967125No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT121150.1752310511447585No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG114370.16542447642943484No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA111320.1610129642049898No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC104960.15181387641893399No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG103770.1500926634526751No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG101570.14691058906127213No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC98300.14218086939768682No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC96000.1388541552612201No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC88640.12820867002452657No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA80640.1166374904194249No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG80070.11581304387256139No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG79720.11530680476483819No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC79340.11475717373359585No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT79100.1144100383454428No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC78800.1139761191102515No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC78450.11346988000252829No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC77990.11280453717523496No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT76790.1110688602344697No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT76160.11015762984056794No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGTC73310.10603539710625047No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG72510.10487827914574031No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT71380.1032438500265197No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA71030.1027376109187965No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT70860.10249172335218809No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA70730.10230369168360519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA21500.069.144761
CCAGACG12250.063.3208966
GTTACGA23400.062.8897064
GTGCGTC8100.059.959479
TTACGAC24600.059.8219155
CCTGGCC52650.059.4818885
GTCCAAT8450.058.357421
CTAGCAT13300.057.7960431
CTCAGAC8950.055.9074861
AACGTTG57400.051.655563
GACAGGC8950.051.028985
GGGCATA6200.050.2777864
CAAACGT59050.049.9825481
TGGCCCG62900.049.7852947
AAACGTT59750.049.504022
ATACGCA15350.048.6403668
CGTTGAG61400.048.4077455
AGAAACT37950.048.3297353
TACGCAA15450.048.325549
CGGGCAT6500.047.9579663
Produced by FastQC (version 0.11.5)