FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_C1bD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_C1bD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660828
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT130980.2810230285262619No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG120780.25913850500383195No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC102960.2209049550852338No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGT94520.2027965846411839No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC93730.20110160683895653No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC86700.18601844994065433No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC82490.17698572013384747No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG73160.1569678177353895No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC71770.15398551502007796No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC71160.1526767346917758No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA70660.15160396393087236No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT65590.1407260684153116No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC64430.13823724025001566No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC63830.13694991533693154No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG63300.13581277833037392No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA60520.12984817289975087No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC60510.12982671748453278No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA56730.12171657053210289No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC55660.11942084110376953No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT52590.1128340286318225No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC51190.10983027050129292No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC50920.10925097429040505No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT50430.1081996589447197No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT48310.10365111091848915No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAGACAACGACCCGTC48270.10356528925761688No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG48120.10324345802934586No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT47860.10268561723367609No Hit
CCGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCCCGTCCGGGGGCCC47190.10124810441406548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA10200.053.305494
TTACGAC10200.053.305495
GTGCGTC6550.052.015749
ATACGCA3800.047.6907968
GAAACTA40100.045.919824
CTTGTTA11700.045.2424051
CGGGCAT6800.044.7766083
AGAAACT41200.044.517843
AAGAAAC42400.044.1146972
AACGTTG18000.043.0945243
GAGTCTA22050.042.7429242
AGTCTAA21600.042.624653
AAACGTT18600.041.316392
TACGCAA4250.040.935549
GGGCATA8150.040.9176864
CAAACGT18850.040.7758641
GCGATAT1451.927765E-840.0065081
CGTTGAG19450.039.881825
GGAGTCT24050.039.497071
CCGGCAT5700.039.4362451
Produced by FastQC (version 0.11.5)