FastQCFastQC Report
Wed 14 Nov 2018
HFVL2BGX9_n01_C1aD14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVL2BGX9_n01_C1aD14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6117316
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA127510.20844108756193075No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT127080.20773816490761635No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA120470.1969327724773414No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT108590.17751249077209677No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG108370.17715285592570335No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG93560.15294289194803734No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC80170.1310542074334561No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT75990.12422114535198116No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA74540.12185082477347908No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC73960.12090269654207826No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC73850.12072287911888155No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC69970.11438022819157945No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG69230.11317054734461976No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC68850.11254935988266748No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC67920.1110290853047317No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG66300.10838086507219834No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT65640.10730196053301806No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG65550.10715483718676622No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG65260.10668077307106581No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA62890.10280652495310033No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA62730.1025449723375415No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCGCGCGGCGC62130.1015641500291958No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG61240.10010926360514971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA20800.068.327731
CTAGCAT14600.065.0612951
GTGCGTC6250.064.947619
CTCAGAC6000.064.051441
CGCGGAT2950.063.9080541
GTCCAAT8550.058.51771
TTACGAC24250.057.9932025
ATACGCA16650.057.031168
GTTACGA24800.056.9993674
TACGCAA16800.056.090499
CCAGACG9750.053.531756
CCGGCAT6000.053.174781
CCTGGCC35250.051.412415
CTCACCC18000.051.1488762
GGTCTGG6250.048.714293
GACAGGC7300.047.665655
CAGGCGT7450.045.7295347
AGTCTAA12950.044.2226143
GAGTCTA17450.042.3749922
GAAACTA35950.041.9422344
Produced by FastQC (version 0.11.5)