FastQCFastQC Report
Thu 8 Aug 2024
HFTWNDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFTWNDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373840846
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTACA112558833.010875649473573No Hit
AATCTCTC106983232.86173196815417No Hit
CGTAAGGA104885022.8056062124361874No Hit
CCACGCGT102932302.753372219792163No Hit
CATGGCCA99495922.6614512850743974No Hit
TAACCGCG97698722.613377351494652No Hit
ATGTCAAT91992862.4607492997167033RNA PCR Primer, Index 15 (100% over 8bp)
CCATCTTA90826772.429557149033415No Hit
CTGACGTG89480222.3935378104724276No Hit
AACCGTGA82633892.210402926383277No Hit
TGGCGGTC78091572.0888988144436205No Hit
AGTTGGCT77776782.0804783862488905No Hit
ACCTAGTA69100251.848386839997682No Hit
TTCCACGA65315351.7471432214766602No Hit
TATGCAGT60624401.6216633535009708No Hit
ATAGAGAG60431671.6165079510867573ABI Solid3 Adapter B (100% over 8bp)
TACTCCTT60100631.6076528459386163ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TCAGGCAA56048621.4992642082775514No Hit
TTCTTCCG52391841.4014477166039798No Hit
CTGTTGGA52184571.3959033786265291No Hit
TCTACTCT51798431.3855743842394364No Hit
AGAGGATA51364271.3739608860183243No Hit
TTCCGTCT50495601.3507245273032578TruSeq Adapter, Index 14 (100% over 8bp)
TCTCAGTG50040571.3385527701272109No Hit
TTGGTGCT49762941.3311263478148667No Hit
CAGTTCCT48779691.304825048464608No Hit
GGAGTTCC48175471.2886625556159799RNA PCR Primer, Index 1 (100% over 8bp)
CACGACAT47210821.2628587941939335No Hit
CCAATGCT46488531.2435380054751963No Hit
CTCCTTAC45366401.2135217562609517No Hit
CACTCACG45067231.2055191529285165No Hit
TTCTCTGC43283201.1577975083011662No Hit
TGAACCGA41011681.0970358225649852No Hit
GTCTGAGG40471041.0825740534516124No Hit
ATGCCTCA40430181.0814810749706039No Hit
TTCAGCAC38209201.0220713014329097No Hit
CACCTACA38200311.021833499702705No Hit
CCTGAACT38142751.0202938070603447No Hit
TTCACGCA37542501.0042375091351041No Hit
AGATATCC35971370.9622108013312168No Hit
GTTAGTGC34924610.9342106507002715No Hit
GGGGGGGG34306340.9176723294703865No Hit
GGTACCTT34284710.9170937410087072No Hit
CTCGATGA33775720.9034785888538246No Hit
TCCAGAGA33117790.8858793883641062No Hit
TGTTACGG32630230.8728374748007071No Hit
AGGCTTAG32204610.861452416036957No Hit
CAGATAGT30839130.8249267122619341No Hit
CTCTAGCT30605440.8186756564316142No Hit
GCATCCAT29764700.7961864070893955No Hit
GCTCACAA28604300.7651464602131786No Hit
TTATGGCC27727060.7416808595602205No Hit
TGCAACCT27227030.7283053815901112No Hit
CGTACGAT26728580.714972167594549RNA PCR Primer, Index 22 (100% over 8bp)
GCGATCTA24922230.6666534774533439No Hit
ACACGCAG24523520.6559882437244431No Hit
GCATTGTC24469310.6545381614078628No Hit
GAGGCTAT24323600.6506405134766895No Hit
ACTCGCTA23484450.6281937956025276No Hit
ACGGTGTC23300900.6232839522303029No Hit
TAGCACAG22107430.591359404317205No Hit
AGATCTCG21671070.5796870575239389Illumina Single End PCR Primer 1 (100% over 8bp)
GGACGTAT20232190.5411979513870455No Hit
GGTCTATG19751720.5283456907220887No Hit
GAGTAGCC19245280.514798749412203No Hit
GGCTAACA18929870.5063617366198663No Hit
GTTCCTAG17838890.4771787296886226No Hit
GTTAACCG15153210.40533853275091297No Hit
TGGTTCAC14800250.3958970818293087No Hit
GAAGGACT14110980.3774595566799033No Hit
GTCGGTAA13598170.36374222200428036No Hit
GACTTCGA10265580.2745976024246425No Hit
CTCGTCGA8999680.2407355990201242No Hit
GAAGTCAG8988260.24043012143194217No Hit
GACCATTG8660940.23167452386944365No Hit
TAGTAACG7544820.2018190382545839No Hit
AGTGTAGA6767570.18102810520603196Illumina Single End PCR Primer 1 (100% over 8bp)
TATCTGAC6741400.18032807469090736No Hit
TATAGTAG6570300.17575126073837313No Hit
ACACGATC5769810.15433867277306557No Hit
GTGACTCT5286580.14141258389940622No Hit
CCTGGCCA5014670.1341391678746629ABI Solid3 GAPDH Forward Primer (100% over 8bp)
TGCTGCTT4832610.12926918103539708No Hit
CACTAGAC4794960.1282620679710317No Hit
CGAGACGT4748590.12702170056612808No Hit
CATACTAG4684050.1252952974539331No Hit
GTAGATCG4263330.11404131050998102No Hit
ACGCTATC4242550.1134854589966341No Hit
TCACGCGT3932590.10519423016713374No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)