FastQCFastQC Report
Thu 8 Aug 2024
HFTWNDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFTWNDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373840846
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATGC120899783.2339906485231955No Hit
GCTACGCT118898793.180465464707406No Hit
ACAGATTC115759203.0964834698667465No Hit
CGAGGCTG103884152.778833589521676No Hit
CCTAAGAC100206382.6804556289710515No Hit
AGCACCTC96244962.5744902150151883No Hit
ACTCGCTA89896122.40466286554466No Hit
ACTGAGCG87338632.336251667908969No Hit
ATCATTCC86735102.3201076321125167No Hit
ACACGACC85882722.2973070203249004No Hit
CAGAGAGG85227262.2797738907321006No Hit
TGGCTTCA83353972.2296645990363504No Hit
CGATCAGT83109492.223124917708965No Hit
AATCCGTC81690962.185180160864498No Hit
CTCTCTAC80536192.1542908128342937No Hit
AAGGACAC77791112.080861704448422No Hit
TAGCGCTC77172352.064310276036557No Hit
TTCACGCA74044281.9806364337191766No Hit
GCTCATGA73479831.96553776255899No Hit
TCTTCACA71765841.91968964247422No Hit
GGGGGGGG63980781.7114443401404027No Hit
ATCTCAGG63020951.6857695105900758No Hit
TATCAGCA62586471.6741474525766507No Hit
TCCGTCTA61610821.6480494482938337No Hit
TAGGCATG57191921.5298467412520247No Hit
GGACTCCT57081411.5268906704753176No Hit
CGGAGCCT54448621.4564652467108958No Hit
CTCGTTGT54434231.4560803235503057No Hit
AAGAGGCA53459071.4299954264494683No Hit
GGAGCTAC50801161.3588980589884498No Hit
AGGCAGAA50677651.3555942466490138No Hit
GCGTATAC49673611.3287368282918983No Hit
TACGCTGC49147611.3146666696768603No Hit
CGAAGTAT46293261.2383146597094958No Hit
CTCGACTT45943431.2289569342564564No Hit
ATGCGCAG45799341.2251026202738693No Hit
GTAACGAG43365881.1600091446401233No Hit
TCCTGAGC41963301.1224910399437733No Hit
GCGTAGTA41721461.1160219769029733No Hit
CTAATGGC40465491.082425594553678No Hit
TGCTCGTA39486461.0562371774645514No Hit
CCTTGAAG38226461.02253299523081No Hit
CACCTATC37849531.0124503623662355No Hit
ACCAAGGA37176380.994444036754614No Hit
GTCTTGTC36258610.9698942849064706No Hit
CGTACTAG35198340.9415327505437968No Hit
TAAGGCGA34444720.9213739046588825No Hit
GGTAACGT33781690.9036382824791702No Hit
GAATCCAG30808100.8240966799010507No Hit
TTGGAGAG30757580.8227453026895836No Hit
GCCATACA30644230.8197132637560958No Hit
CAACAGAG29738330.7954810267040751No Hit
GATCTACG28835470.7713301076790309No Hit
GTAGAGGA24175640.6466826795058129No Hit
TCATTGCG19755630.5284502806844172No Hit
CAACCGAG8813540.23575647482886342No Hit
TAACGTCC8648950.2313537991512035No Hit
CTACGACC8619600.23056870569996518No Hit
GTTACAGC6391420.17096633683522106No Hit
ACGTGCGC5643750.1509666495886327No Hit
ATAGTACC5628000.15054534731071095No Hit
ACGTACGT5328270.1425277643417274No Hit
TAGCAGCT5034430.13466773505001112No Hit
CGCGATAT4747790.12700030108534474No Hit
TACTAGGT4049090.10831052955620586No Hit
CTATCGTG3872910.10359782890069749No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)