FastQCFastQC Report
Thu 8 Aug 2024
HFTWNDRX5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFTWNDRX5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373840846
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTCGGATTGACTCG58870151.5747383045457797No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTAGTAACAAGGATTCCGTAGGTG14194980.37970650216215274No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGATTCCGTAGGTC8499710.2273617260110737No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGATTCCGTAGGTG8360960.22365025356271528No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTCGGATTGTCTCG8121710.21725047134095132No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTCGGATTGACTCT7689460.20568806438020953No Hit
GGCTTGGCATCGATGAAGAACGCAGCCGCTGTCTCTTAAACACATCTCCG7571960.20254501564015828No Hit
GGCTTGGTCATTTAGAGCAAGTAAAAGTAGTAACAAGGATTCCGTAGGTC7383610.19750677538323355No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTG7065190.1889892470444495No Hit
GGCTTGGTCATTTAGAGGAAGAACGCAGACGCTGTCTCATATACACATCT6898580.1845325376778117No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTG6840740.1829853552171771No Hit
GGCTTGGTCATTTAGAGGAAGAACGCAGACGCTGTCTCATATACACATCA5100950.13644710187714482No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTCGGATTGTCTCA4390700.1174483753441966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)