FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n02_wtndb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n02_wtndb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28074567
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8412002.996306229763045No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1251450.4457593237324016Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT226650.028.04226537
ATCTCGG231900.027.33072736
AGATCTC233250.027.17680234
CTCGGTG232600.027.1504938
TCCTAGG1400.025.71114540
GATCTCG245800.025.66722935
GGTCGCC253550.025.5611144
GACCTAG3700.024.9357424
GTCGCCG261650.024.48529845
GTGGTCG267850.024.06293142
TCGGTGG267400.024.0289539
GGTGGTC272950.023.53935241
TAGATCT426150.022.19676445
TGGTCGC298550.021.69359843
AGTGTAG473700.020.38335841
GTGTAGA486150.019.80564742
CGGTGGT333200.019.56693640
GAGTGTA619300.019.26052740
TGTAGAT514850.018.76239843
AGAGTGT664450.018.2128239
Produced by FastQC (version 0.11.2)