FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n02_wtdb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n02_wtdb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32430816
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8207992.530923057871871No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2715140.8372098932077441Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT390700.032.72527737
ATCTCGG396750.032.28962736
CTCGGTG404600.031.61724738
GATCTCG413050.030.95676835
GGTCGCC426500.030.63400344
TAGATCT1244600.030.05451645
GTCGCCG434650.029.89240845
AGATCTC437450.029.28448134
TCGGTGG447550.029.20583339
AGTGTAG1341550.028.53290741
GTGTAGA1353050.028.23881342
GAGTGTA1355800.028.23528540
GTGGTCG466050.028.06968342
GGTGGTC470700.027.78286641
TGGTCGC478700.027.40713543
AGAGTGT1404000.027.32874339
TGTAGAT1422050.026.95971543
AAGAGTG1456500.026.42602238
AAAGAGT1513200.025.47930337
GGAAAGA1581450.024.4639935