FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n02_tusndb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n02_tusndb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26744227
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9234573.452920886440277No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG366380.13699405108997914Illumina Single End PCR Primer 1 (100% over 51bp)
GGGCATATACCCCGGTAATAATCAGCTGGTTACGTCCACTCTCTTTCAGCA289270.1081616604585356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAC55150.025.6165394
GTCCACT56200.023.8239934
TACGTCC57950.023.49461431
GCTCCAA29150.023.31404733
TACCCCG59100.023.0298168
CACTCTC60000.022.6856437
TCCACTC60550.022.1471235
ATACCCC63600.021.89547
ATATACC65000.021.2853245
AGATCTC92300.020.94234734
CAAGTCT34150.020.88889325
TATACCC66750.020.6936176
GCATATA70400.020.3242033
ACGTCCA70150.019.98592832
CGTCCAC69400.019.94182833
CCACTCT68750.019.76639436
CCCCGGT69650.019.67105710
ACTCTCT70250.019.59970738
ACCCCGG70600.019.5336049
AGTCTTG35300.019.3806227
Produced by FastQC (version 0.11.2)