FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n02_tusdb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n02_tusdb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28354955
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7790032.747325820125618No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1712420.6039226653683634Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT288600.028.01779637
ATCTCGG292900.027.6073536
GGTCGCC301000.027.11531844
CTCGGTG300250.027.01262538
AGATCTC301250.026.88293634
GTCGCCG307300.026.59471145
GATCTCG304250.026.49719235
TCGGTGG337600.024.33715839
GTGGTCG350050.023.50296242
TAGATCT351350.023.20447733
TGGTCGC355750.023.17040643
GGTGGTC356100.023.05319441
AGTGTAG415500.019.9098429
GTGTAGA419050.019.75728630
CGGTGGT433900.019.24658440
GAGTGTA435100.019.06478128
TGTAGAT463950.018.07300631
AGAGTGT476350.017.47038727
AAGAGTG526350.015.87910326
GTAGATC579400.014.53391132
Produced by FastQC (version 0.11.2)