FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n01_wtndb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n01_wtndb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28074567
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1207020.42993361215508685TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG181500.035.8970844
CTCGTAT175300.035.53666343
TCTCGTA173200.035.38314442
GCCTAGG4650.033.38361444
CGTATGC200900.033.3713345
ATCTCGT182250.033.1819641
CCTAGGC5600.032.5412545
GATCTCG206650.029.42817140
ACGATCT242200.025.49950238
CACGATC248150.024.78897537
TCACGAT265100.024.13790736
ATCACGA279000.023.21817435
GAGGGCC10750.022.6036221
CGATCTC279100.021.9021339
TACACGA116900.021.6894313
GTCTAGT2500.021.60143925
GTCACAT510450.020.04051842
GTCCTAG1351.9663275E-920.0026863
CCCTACA129650.019.90354510
ACACGAC127800.019.7515314
Produced by FastQC (version 0.11.2)