FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n01_wtdb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n01_wtdb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32430816
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2687800.8287796397105764TruSeq Adapter, Index 2 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT347200.039.18957543
TCGTATG353850.039.05078544
TCTCGTA355400.038.08913442
CGTATGC380000.036.60621645
ATCTCGT377650.035.88078341
GTATCTC385450.035.1327639
TGTATCT387350.035.04228238
ATGTATC391650.034.76771537
TATCTCG396800.034.18415540
GATGTAT420350.032.94531636
CGATGTA450850.030.9367735
AGTCACC1446850.026.7854841
ACCGATG1458900.026.48100945
CCGATGT531000.026.26527434
CACCGAT1483800.026.10485344
GTCACCG1492950.025.9071542
CAGTCAC1502150.025.8465940
TCACCGA1510650.025.6676443
TCCAGTC1580300.024.63881338
CCAGTCA1580700.024.59234439
Produced by FastQC (version 0.11.2)