FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n01_tusndb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n01_tusndb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26744227
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC356920.13345683911522288TruSeq Adapter, Index 3 (100% over 50bp)
GTCAACAGCGCAGGCTGAGTTTGCCAGGTTTTATTCAGTTCTTCAGCTGGC328360.12277789894619127No Hit
CCGACGACACGGTGCTGGTGAAGTGGCGCTACAGCGCGTTTCATCGTTCTC302600.11314591369569216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC62100.030.25611734
CTCGTAT64950.028.71551143
TCTCGTA63700.028.53742242
TCGTATG71450.026.32359744
TTAGGCA72500.025.85206835
CTCTTGG30550.025.40703218
TAGGCAT74900.024.84294336
GACACGG58250.024.2602376
ATCTCGT77850.023.5527641
GGCTGAG74350.021.30257213
CGTATGC95700.020.54663545
GCTGAGT79200.020.50937514
ACTTAGG114100.020.26976245
GGGCACT39950.019.76654213
TCTTGGC41350.019.6424119
TAGGTCC12050.019.42097932
CATCTCG94850.019.2844940
CGACACG73250.019.2614775
CACTTAG124950.018.65368744
ACGACAC78400.018.2545344
Produced by FastQC (version 0.11.2)