FastQCFastQC Report
Tue 8 Nov 2016
HFNNLAFXX_n01_tusdb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNLAFXX_n01_tusdb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28354955
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1644080.5798210577304743TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG227650.037.227944
CTCGTAT226350.035.80171243
CGTATGC247350.034.44482445
TCTCGTA244000.033.0829342
ATCTCGT263500.030.48962841
AATCTCG283450.028.4394340
CCAATCT291150.027.62606238
CAATCTC326050.024.81360439
GACCAAT347250.024.35578236
TGACCAA370650.022.91579235
ACCAATC368350.022.05042537
AGTCACT460700.018.702629
CACTGAC460050.018.67974932
CTGACCA463850.018.59905834
ACTGACC459200.018.59178433
GTCACTG468500.018.3718830
TCACTGA468300.018.36027731
CAGTCAC517350.016.79366928
TCCAGTC599400.014.76784826
CCAGTCA598800.014.71928427
Produced by FastQC (version 0.11.2)