FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14291263
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG327400.22909101875740445RNA PCR Primer, Index 26 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT61200.056.7057542
TCGTATG64000.056.59654243
TATGCCG68250.053.5278746
ACGTCTG97200.051.6771415
AGTCACA86500.049.61473528
ACACGTC102700.049.2768913
CGTATGC74100.049.20818744
GTATGCC75600.048.96192645
GTCACAT84950.048.8551329
CACGTCT103900.048.60951614
CAGTCAC92000.047.17329427
GTCTGAA108750.046.34874717
ATGCCGT81100.045.2592647
CACACGT112650.044.77115212
TCCAGTC117900.042.1938125
CCAGTCA110600.041.64121626
ACATGAG98550.041.48237232
CACATGA100050.041.30870431
CTCCAGT123950.040.44048724
TCTGAAC125200.040.3967818
Produced by FastQC (version 0.11.2)