FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6106
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG110.1801506714706846TruSeq Adapter, Index 27 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.002244548469.013
CACACGT150.002244548469.012
CACGTCT150.002244548469.014
CTCCAGT150.002244548469.024
GATCGGA150.002244548469.01
TCCAGTC150.002244548469.025
AACTCCA150.002244548469.022
CGGAAGA150.002244548469.04
CTGAACT150.002244548469.019
GAACTCC150.002244548469.021
AGCACAC150.002244548469.010
TGAACTC150.002244548469.020
GCACACG150.002244548469.011
CGTCTGA150.002244548469.016
ACGTCTG200.007014875351.7515
CCAGTCA200.007014875351.7526
AAGAGCA200.007014875351.757
ACTCCAG200.007014875351.7523
GTCTGAA200.007014875351.7517
GAAGAGC200.007014875351.756
Produced by FastQC (version 0.11.2)