FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12816609
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG326200.25451349885137325RNA PCR Primer, Index 28 (100% over 43bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT138930.10839840709816458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51350.055.2899943
TATGCCG51700.054.91675646
CTCGTAT48150.054.88047842
ACGTCTG69650.051.8602615
CAGTCAC64700.047.50917427
GTATGCC61150.046.65496445
CACGTCT78700.046.50982314
ACACGTC79150.046.07068613
GTCTGAA83050.043.70089317
CGTATGC65350.043.39236544
ATGCCGT67400.042.7387847
AGTCACC71200.042.15427428
CACACGT92100.039.6299312
CCAGTCA82400.039.4808826
CCGTCTT73500.038.25422350
CTCCAGT95250.038.02988424
TCCAGTC95400.037.97023425
TCTGAAC96750.037.54841618
GCCGTCT74850.037.4716549
TCTCGTA72400.036.30809841
Produced by FastQC (version 0.11.2)