FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21102
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGT150.002323554169.000017
TCTGAAC308.8208E-657.50000418
ACACGTC308.8208E-657.50000413
CTCCAGT308.8208E-657.50000424
ACGTCTG308.8208E-657.50000415
CACACGT308.8208E-657.50000412
AACTCCA308.8208E-657.50000422
ACTCCAG308.8208E-657.50000423
CACGTCT308.8208E-657.50000414
ATCGGAA308.8208E-657.5000042
CAGTCAC252.5096931E-455.227
CGGAAGA352.1972446E-549.2857134
GTCTGAA352.1972446E-549.28571317
GAACTCC352.1972446E-549.28571321
TCGGAAG352.1972446E-549.2857133
GAGCACA352.1972446E-549.2857139
AGAGCAC352.1972446E-549.2857138
AGTCACA306.170961E-446.00000428
CCAGTCA306.170961E-446.00000426
GAAGAGC404.836385E-543.1256
Produced by FastQC (version 0.11.2)