FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624083
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1182821.1133384405976496RNA PCR Primer, Index 32 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG163750.066.0037946
TGCCGTC163450.065.8089648
GCCGTCT163450.065.6823349
CGTATGC164950.065.56451444
ATGCCGT165700.065.01884547
CTCGTAT147150.064.7270342
TCGTATG167550.064.5882843
TCTCGTA147750.064.25465441
CGTCTTC166650.064.21551
CCGTCTT167300.064.1098550
ATCTCGT149500.063.4572540
GTATGCC177350.060.9803645
AATCTCG155750.060.93382339
ACCACTC207300.052.8531632
CTGCTTG202650.052.79017357
CTCAATC184650.052.14523736
GTCACCA213450.051.4287629
AGTCACC214550.051.1648428
TATCGTA21200.050.60671241
TGCTTGA213650.050.23439858
Produced by FastQC (version 0.11.2)