FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13536004
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG664280.4907504459957311RNA PCR Primer, Index 31 (100% over 43bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT158110.11680699857949214No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG138590.10238619905845181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT97450.059.54265242
TCGTATG100700.058.92296643
TATGCCG101050.058.44598446
ACGATAT103300.058.01020435
TATCTCG100450.057.80021339
GTATGCC106450.055.4813445
CGATATC105100.055.47310336
CACGATA109650.055.02829734
ACGTCTG125350.054.7423415
CAGTCAC113800.054.32611527
CCACGAT111600.054.25265533
CGTATGC112050.052.92363444
ATGCCGT112900.052.4337147
CACGTCT134400.051.10677314
ATATCTC114600.050.69373738
AGTCACC121700.050.4879628
ACACGTC136850.050.3176813
GTCTGAA137400.049.9667217
GATATCT118600.049.21688537
ACCACGA124750.048.83786432
Produced by FastQC (version 0.11.2)