FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14246161
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG290880.20418132295430325RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT49800.051.26070842
TATGCCG56450.050.84493646
TCGTATG57250.050.79709243
ACGTCTG82150.049.9749215
CACGTCT88250.046.5977714
CGTATGC62350.046.53142544
ACACGTC90900.045.3142613
GTATGCC67600.042.815645
CAGTCAC75950.042.56134827
GTCTGAA99900.041.26859717
ATGCCGT72250.040.39436747
AGTCACC79550.039.59485628
CACACGT105900.038.99312612
TCCAGTC108700.037.32354425
CCAGTCA95400.036.9939626
TCTGAAC114800.035.9423618
CTCCAGT114900.035.42954324
GCCGTCT84000.034.25174749
CCGTCTT83150.034.18699350
TCTCGTA74400.033.89465741
Produced by FastQC (version 0.11.2)