FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9w-43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9w-43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14737679
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG674830.4578943536495808RNA PCR Primer, Index 29 (100% over 43bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT166950.11328106684912868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT93450.059.17551442
TCGTATG110500.058.84870543
TATGCCG111250.058.5147846
GTATGCC121950.053.32370445
ATGCCGT122500.052.8595447
CAGTCAC127950.052.82122427
CGTATGC123550.052.66071344
TAATCTC105400.052.23846438
CTAATCT106900.051.66682437
AGTCACC130550.051.1878728
ACGTCTG144150.050.9775915
CACGTCT152600.048.22194714
GCCGTCT134600.048.00564649
GTCTGAA153950.047.9121117
CCAGTCA147100.047.5156526
ACACGTC156950.047.0383513
CCGTCTT139200.046.5927450
TCCAGTC159000.046.12921525
ACTAATC121150.045.87469536
TCTCGTA119600.045.83354641
Produced by FastQC (version 0.11.2)