FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12561021
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1272981.013436726202432TruSeq Adapter, Index 18 (97% over 40bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG157550.12542770209523574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT155050.064.10020444
TCGTATG163250.063.69123545
TATGCCG164950.063.0355848
ACGTCCG167200.062.93109532
GTCACGT170050.062.22126829
TCACGTC171850.061.5098130
CGCACAT171200.060.91642437
CCGCACA172850.060.73457736
CGTATGC171700.060.69787646
AGTCACG175950.060.66428
GTATGCC172350.060.3288547
GTCCGCA174450.060.09867534
CAGTCAC182400.058.82142627
ATGCCGT176900.058.7773849
ACGTCTG201200.056.91104515
TCCGCAC185450.056.75737435
CCGTCTT182650.056.341552
GCCGTCT184700.055.9965551
CCAGTCA199100.054.71912426
TCTCGTA181450.054.7169343
Produced by FastQC (version 0.11.2)