FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623591
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA670430.5310929354412702TruSeq Adapter, Index 20 (97% over 44bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG212600.16841483536657675No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG136040.10776648261180198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82050.060.50157544
TATGCCG88500.058.9383848
TCGTATG88900.058.8667545
AGTCACG92050.058.7654828
GTCACGT91850.058.1800429
TATCTCG86050.057.5297441
TCACGTG93650.056.76753230
GCCTTAT91200.056.47591437
ACGTCTG114550.056.0479715
GTATGCC96150.054.7507147
CAGTCAC101250.054.2090427
CTTATCT92000.053.73534839
CCTTATC92900.053.25148438
CGTATGC98550.053.24275646
CACGTCT121650.052.86017614
TTATCTC93650.052.7875540
ATGCCGT99950.052.49740249
ACACGTC124850.051.47668513
GTCTGAA127600.050.45157217
CACGTGG107500.049.58193231
Produced by FastQC (version 0.11.2)