FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367500
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA583210.4715666060238528TruSeq Adapter, Index 7 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG140610.1136931473620376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT78750.058.13103544
CGTATGC83800.056.2343146
TATGCCG84000.055.8549148
TCGTATG84750.055.48116745
CACGAGT85600.055.33504531
TATCTCG85850.053.0022141
TCACGAG92250.051.79509730
AGTCACG93950.051.29914528
ACGAGTG93950.050.4533432
GTATGCC94400.049.7736947
ACGTCTG111100.049.15688715
GTCACGA97650.049.1082929
ATGCCGT97350.048.15986649
GTGGATA98050.047.85141836
AGTGGAT98800.047.55839535
CAGTCAC103300.047.55724327
CACGTCT117500.046.56711614
ACACGTC118900.046.2209813
ATATCTC99150.046.10198640
TCTCGTA100250.045.52638243
Produced by FastQC (version 0.11.2)