FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13277051
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA743170.5597402615987541TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC91500.061.64472638
CTCGTAT95600.059.25200744
TATGCCG103950.059.1060848
TACGTAA105500.058.72951536
TCGTATG104550.058.70022245
ACGTAAT105750.058.1987637
CCGTACG109300.056.4989433
CGTATGC112700.054.70034446
CAGTCAC117350.054.651527
GTATGCC112950.054.60981447
ATGCCGT116550.052.80502349
ACCGTAC118400.052.7971732
TAATCTC107550.052.57281540
ACGTCTG136600.051.21882215
AGTCACC129550.048.89236528
CCGTCTT125700.048.6867852
GCCGTCT128750.047.42624751
CACGTCT148050.047.41984614
GTCTGAA148600.047.31576517
CCAGTCA140450.046.9152626
Produced by FastQC (version 0.11.2)