FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12561477
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG349470.27820772987125636RNA PCR Primer, Index 24 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55050.055.20884343
CTCGTAT52950.054.59691642
AGTCACG59350.053.71085728
TATGCCG57900.052.79006246
CGTATGC60000.050.884144
ACGTCTG79650.047.21190615
CAGTCAC73200.045.0092127
CACGTCT84050.044.90402214
GTATGCC68350.044.819645
GGTAGCA70500.043.7475834
ATGCCGT71350.042.9352847
GTCACGG75650.042.09239229
TAGCATC67900.041.9166336
ACACGTC90950.041.76219613
CCAGTCA83050.041.4989126
TCTCGTA73050.039.10226441
CCGTCTT79300.038.7618950
GTCTGAA97250.038.70373517
GTAGCAT81050.038.22321735
TCCAGTC97300.038.0450125
Produced by FastQC (version 0.11.2)