FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_9s-43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_9s-43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13742406
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA372330.270935089532357TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG67800.058.77094333
ACGTTTC71100.056.23685532
CTCGTAT57400.055.8932444
TTTCGGA71850.055.79509735
GTCACGT72850.055.69059429
TCGTATG65300.055.36533445
TTCGGAA72850.054.69691536
CACGTTT74150.054.15630331
TATGCCG67250.053.70982448
AGTCACG77850.053.17715528
TCGGAAT76750.051.87182237
ACGTCTG100700.049.1960915
GTATGCC75200.048.62774747
CAGTCAC87500.048.4556827
CGTATGC77500.046.96203646
CACGTCT107600.046.13683714
ATGCCGT80350.044.61031749
CCAGTCA100700.044.36442626
ACACGTC112850.044.29568513
GTCTGAA115100.043.1314717
Produced by FastQC (version 0.11.2)