FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13878965
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG337200.24295759806296796TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT56950.055.3058142
TCGTATG60550.053.78380643
TATGCCG62200.053.02262546
TATCTCG62900.050.67912739
ACGTCTG85250.049.85856215
AGTCACT73150.049.6142128
CGTATGC68050.048.16012644
GTATGCC70750.046.7613945
CACGTCT91550.046.53943314
ACACGTC92750.046.12162813
CAGTCAC81950.045.591227
CTTATCT74300.043.59980837
ATGCCGT75750.043.49250847
TTATCTC75250.042.774338
GTCTGAA102250.041.40041417
CACACGT105550.040.52789312
CCAGTCA98050.039.9346426
TCACTAG91700.038.86307530
TCCAGTC108200.038.70688625
CTCCAGT111650.037.603424
Produced by FastQC (version 0.11.2)