FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072475
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG527790.35016810444203755TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80950.054.6337643
CTCGTAT77400.054.46515742
ACGTCTG111700.053.9874215
TATGCCG85450.052.03990646
AGTCACG92100.051.80425328
CGTATGC89050.049.62552644
CACGTCT121900.049.55425614
CACGGCT92050.049.47208431
ACACGTC125000.048.35259213
GTATGCC93150.047.7378545
CAGTCAC104350.047.70609727
GCTACAT95400.046.28911635
GTCTGAA133050.045.35125717
ATGCCGT98200.045.2479247
CACACGT134850.044.76925712
GTCACGG108350.043.30277629
CCAGTCA125200.043.20548226
TCCAGTC142350.041.87775425
TCTGAAC144950.041.7708518
TCTCGTA104150.040.31091341
Produced by FastQC (version 0.11.2)