FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13933604
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG584180.41925979811109887TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82200.058.71275742
TCGTATG91650.058.23010343
TATGCCG92850.057.29217546
GTATGCC100550.052.93854545
ACGTCTG118900.052.89464615
CAGTCAC108250.052.1068927
TAATCTC94000.051.30702638
ATGCCGT104450.051.06153547
CGTATGC104900.050.74328644
TGTAATC94850.050.70284336
CTTGTAA107400.049.82193834
CACGTCT127750.049.14841514
ACACGTC129800.048.63762313
TTGTAAT110400.048.28023535
AGTCACC115050.048.18803828
GTCTGAA135250.046.60355417
GCCGTCT115900.045.7203949
TCTCGTA105250.045.65786441
CCAGTCA129400.045.0297126
CCGTCTT118050.044.82909450
Produced by FastQC (version 0.11.2)