FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15654869
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG402520.2571212828417791TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT64150.053.45442642
TCGTATG72200.051.89034343
AGTCACG75100.051.5882128
ACGTCTG88200.050.96620615
TATGCCG75200.049.91248346
CGTATGC75700.049.3543244
GTCACGA79350.048.21645429
CACGTCT98300.045.86953714
CAGTCAC89100.044.91494827
GTATGCC84500.044.41887745
ACACGTC103650.043.867513
ATGCCGT89000.042.17310747
GTCTGAA111600.040.311317
TCACGAT98850.038.60027730
CACGATC98150.038.55910531
CCAGTCA110150.037.9599826
CACACGT119400.037.8784612
GCCGTCT99550.037.73911349
CCGTCTT101800.036.7017850
TCTCGTA95100.035.9128641
Produced by FastQC (version 0.11.2)