FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12899412
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA407220.31568880814102224TruSeq Adapter, Index 15 (97% over 40bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT136950.10616762996638916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59600.057.82434544
TCGTATG65450.056.3984745
TATGCCG69750.053.21874248
CGTATGC75000.049.95263746
GTCACAT81700.049.2358529
ACGTCTG93600.048.21071615
GTATGCC78450.047.79983547
AGTCACA85150.047.76767728
ATGCCGT77700.047.72955349
CAGTCAC89150.046.12677827
CACGTCT100350.045.0699314
ACACGTC102250.044.50167513
CACATGT90700.043.58954231
GTCTGAA107100.042.39209417
CCAGTCA102350.041.89686626
TCCAGTC109200.041.1323225
CACACGT110800.040.88036312
CCGTCTT89000.040.46818552
ATGTCAG98650.040.14700334
TCTCGTA86100.040.02707343
Produced by FastQC (version 0.11.2)