FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509334
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA537910.3981765496359776TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT68250.059.44209339
CTCGTAT70750.057.146244
TCGTATG74050.056.60273745
TATGCCG76150.055.26891748
TATCTCG74050.054.64650341
TTCCGTA79650.053.36080636
AGTTCCG84850.050.57887634
ATGCCGT84750.049.82369649
GTATGCC85300.049.5425247
ACGTCTG106200.049.27934615
CGTATGC86600.048.7591546
AGTCACA93100.048.6905628
CAGTCAC94150.048.62352827
TCCGTAT87650.048.25340337
CCGTATC85300.047.9650738
GTCACAG92900.047.755629
GTATCTC88950.045.5314640
CACGTCT115600.045.45042414
GTTCCGT95600.044.42211535
ACACGTC119850.044.18346413
Produced by FastQC (version 0.11.2)