FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083872
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG420770.32159440263555006TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65650.056.3310142
TCGTATG72700.056.2306543
TATGCCG74050.055.53162446
ACGTCTG93500.051.4355715
CGTATGC80650.050.8160944
GTATGCC83100.049.56698245
ATGCCGT85150.048.33313847
CACGTCT101000.048.0586814
ACACGTC101650.047.445213
CAGTCAC91550.047.14272727
GTCTGAA109250.044.17894417
GCCGTCT94300.043.24125749
CCGTCTT94750.043.1813650
CGTCCAT96100.042.36098535
AGTCACC101300.042.19661328
CACACGT114450.042.13880512
CCAGTCA106800.041.15386226
TCTCGTA90850.040.0225641
TCCAGTC118700.039.87592725
CTCCAGT119800.039.74001324
Produced by FastQC (version 0.11.2)