FastQCFastQC Report
Mon 6 Mar 2017
HFNNGBGX2_n01_4w-43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFNNGBGX2_n01_4w-43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13861816
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA444740.32083819320643125TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT62050.055.4295944
TCGTATG68700.054.8349345
TATGCCG71150.053.3852248
ACGTCTG92600.050.4064415
CAGTCAC84200.048.756527
AGTCACA84550.048.10601828
CGTATGC79300.047.7673946
GTATGCC80950.047.00683647
CACGTCT100350.046.51356514
GTCACAG85900.046.14531729
ATGCCGT83050.045.943549
ACAGTCA85750.045.060332
CACAGTC87650.044.35856231
ACACGTC108100.043.6250313
GTCTGAA107550.043.52846517
CCAGTCA100650.042.60399226
TCCAGTC110200.042.04253825
CACACGT113800.041.0758812
TCTCGTA86000.040.1134343
CTCCAGT118800.039.2894424
Produced by FastQC (version 0.11.2)